total src Search Results


pel src y419 t  (RayBiotech inc)
90
RayBiotech inc pel src y419 t
Pel Src Y419 T, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+src/Human+Phospho-SRC+(Y419)+and+Total+SRC+ELISA/pm34944014-79-52-56
Average 90 stars, based on 1 article reviews
pel src y419 t - by Bioz Stars, 2026-10
90/100 stars
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v-src  (Merck KGaA)
90
Merck KGaA v-src
Tyrosine phosphorylation of CEACAM20 by SFKs and its association with Syk. (A) HEK293A cells expressing Myc-epitope–tagged CEACAM20 (CC20Myc) were treated with 10 μM PP2 or PP3 (or with DMSO vehicle) for 30 min, after which cell lysates were subjected to immunoprecipitation with antibodies to Myc and the resulting precipitates were subjected to immunoblot analysis with antibodies to Myc or to phosphotyrosine (α-pY). (B and C) Lysates of HEK293A cells transfected with an expression vector for CC20Myc together with either an expression vector for <t>c-Src</t> (B), an active mutant of Fyn [Fyn CA] (C), or the corresponding empty vector (B and C) were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to Myc (B and C), to <t>v-Src</t> (B), or to Fyn (C). (D) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to SAP-1. (E) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to CEACAM20 (α-CC20), and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with antibodies to CEACAM20, to Syk, or to phosphorylated Syk (α-pSyk). (F) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to Myc, and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with the indicated antibodies. All data are representative of three independent experiments.
V Src, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+src/total+c+src+antibody/pmc04534239-458-19-22
Average 90 stars, based on 1 article reviews
v-src - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
total src  (GenScript corporation)
90
GenScript corporation total src
Tyrosine phosphorylation of CEACAM20 by SFKs and its association with Syk. (A) HEK293A cells expressing Myc-epitope–tagged CEACAM20 (CC20Myc) were treated with 10 μM PP2 or PP3 (or with DMSO vehicle) for 30 min, after which cell lysates were subjected to immunoprecipitation with antibodies to Myc and the resulting precipitates were subjected to immunoblot analysis with antibodies to Myc or to phosphotyrosine (α-pY). (B and C) Lysates of HEK293A cells transfected with an expression vector for CC20Myc together with either an expression vector for <t>c-Src</t> (B), an active mutant of Fyn [Fyn CA] (C), or the corresponding empty vector (B and C) were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to Myc (B and C), to <t>v-Src</t> (B), or to Fyn (C). (D) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to SAP-1. (E) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to CEACAM20 (α-CC20), and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with antibodies to CEACAM20, to Syk, or to phosphorylated Syk (α-pSyk). (F) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to Myc, and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with the indicated antibodies. All data are representative of three independent experiments.
Total Src, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+src/total+src/pm22543583-121-72-75
Average 90 stars, based on 1 article reviews
total src - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
human phospho-src (y419) and total src elisa  (RayBiotech)
N/A
RayBio® Human Phospho-SRC (Y419) and Total SRC ELISA Kit. This assay semi-quantitatively measures phosphorylated SRC (Tyr419) and Total SRC in lysate samples.
   Buy from Supplier
human phospho-src (y419) and total src elisa  (RayBiotech inc)
N/A
RayBio® Human Phospho-SRC (Y419) and Total SRC ELISA Kit. This assay semi-quantitatively measures phosphorylated SRC (Tyr419) and Total SRC in lysate samples.
   Buy from Supplier

Image Search Results


Tyrosine phosphorylation of CEACAM20 by SFKs and its association with Syk. (A) HEK293A cells expressing Myc-epitope–tagged CEACAM20 (CC20Myc) were treated with 10 μM PP2 or PP3 (or with DMSO vehicle) for 30 min, after which cell lysates were subjected to immunoprecipitation with antibodies to Myc and the resulting precipitates were subjected to immunoblot analysis with antibodies to Myc or to phosphotyrosine (α-pY). (B and C) Lysates of HEK293A cells transfected with an expression vector for CC20Myc together with either an expression vector for c-Src (B), an active mutant of Fyn [Fyn CA] (C), or the corresponding empty vector (B and C) were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to Myc (B and C), to v-Src (B), or to Fyn (C). (D) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to SAP-1. (E) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to CEACAM20 (α-CC20), and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with antibodies to CEACAM20, to Syk, or to phosphorylated Syk (α-pSyk). (F) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to Myc, and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with the indicated antibodies. All data are representative of three independent experiments.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Protein tyrosine phosphatase SAP-1 protects against colitis through regulation of CEACAM20 in the intestinal epithelium

doi: 10.1073/pnas.1510167112

Figure Lengend Snippet: Tyrosine phosphorylation of CEACAM20 by SFKs and its association with Syk. (A) HEK293A cells expressing Myc-epitope–tagged CEACAM20 (CC20Myc) were treated with 10 μM PP2 or PP3 (or with DMSO vehicle) for 30 min, after which cell lysates were subjected to immunoprecipitation with antibodies to Myc and the resulting precipitates were subjected to immunoblot analysis with antibodies to Myc or to phosphotyrosine (α-pY). (B and C) Lysates of HEK293A cells transfected with an expression vector for CC20Myc together with either an expression vector for c-Src (B), an active mutant of Fyn [Fyn CA] (C), or the corresponding empty vector (B and C) were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to Myc (B and C), to v-Src (B), or to Fyn (C). (D) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation and immunoblot analysis as in A. Total cell lysates were also subjected to immunoblot analysis with antibodies to SAP-1. (E) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to CEACAM20 (α-CC20), and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with antibodies to CEACAM20, to Syk, or to phosphorylated Syk (α-pSyk). (F) Lysates of HEK293A cells transfected with expression vectors for the indicated proteins were subjected to immunoprecipitation with antibodies to Myc, and the resulting precipitates as well as the original cell lysates were subjected to immunoblot analysis with the indicated antibodies. All data are representative of three independent experiments.

Article Snippet: Mouse mAbs to β-catenin and to Eps8 were obtained from BD Biosciences, and those to phosphotyrosine (4G10) and to v-Src were from Merck Millipore.

Techniques: Expressing, Immunoprecipitation, Western Blot, Transfection, Plasmid Preparation, Mutagenesis